Fig. 4
Effect of treatment of Caco-2 and HCT-116 cells for 48 h with subIC50 concentrations of metformin, 5-ASA or the combination of both drugs on (a) TNF-α (b) TNF-R1 (c) TNF-R2 and (d) IL-1β gene expression levels and on (e) TNF-α and (f) IL-6 protein levels. All data are expressed as mean ± SD of 3 separate experiments. The statistical significance of the results was analyzed using one way ANOVA followed by Tukey-Kramer multiple comparison test. a Significantly different from control, b from metformin and c from 5-ASA (P <0.05)