Fig. 3
Western blot for Bcl-2 and Bax levels in (a) Caco-2 and (b) HCT-116 cells after treatment with metformin, 5-ASA or their combination. One representative western blot of three independent experiments is shown. Effect of treatment of Caco-2 and HCT-116 cells for 48 h with subIC50 concentrations of metformin, 5-ASA or the combination of both drugs on (c) Bcl-2 level (d) Bax level. All data are expressed as mean ± SD of 3 separate experiments. The statistical significance of the results was analyzed using one way ANOVA followed by Tukey-Kramer multiple comparison test. a Significantly different from control, b from metformin and c from 5-ASA (P <0.05)