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Fig. 2 | BMC Cancer

Fig. 2

From: Anti-c-Met monoclonal antibody ABT-700 breaks oncogene addiction in tumors with MET amplification

Fig. 2

ABT-700 inhibits proliferation and induces apoptosis in MET amplified tumor cells. a MET status and dependence in a panel of 35 human cancer lines. Average of MET/CEP7 was calculated in 20 random cells and the red symbol indicates cell lines harboring MET amplification (MET/CEP 7 ratio ≥2). MET dependence was indicated by the maximal inhibition of proliferation of each cell line grown under regular medium containing FBS by the selective c-Met kinase inhibitor PF-4217903 at 1 μM for 3 days. Additional details are summarized in Table 1. b Image of FISH analysis of MET in SNU5 cells. Red represents signal of MET while green represents CEP 7. FISH images of additional cell lines are shown in Additional file 3: Figure S1. c Immune-blots of total and phosphorylated c-Met protein from total cell lysate of A549 and SNU5 cells as described in the legend of Fig. 1. d and e Inhibition of SNU5 proliferation. SNU5 cells were plated in 96-well plate and treated with antibodies or antibody fragments in a dose range as shown for 3 days. Quantification of live cells at the end of incubation was done with Cell-titer Glo reagents. Data are from one representative experiment. f Immune-blots of signaling and apoptosis pathway molecules in SNU5 cells treated with 10 μg/mL ABT-700 for 24h as described in the legend of Fig. 1. g Dual PI and Annexin V FACS analysis of SNU5 cells treated with 10 μg/mL ABT-700 for 24h. The percent of Annexin V positive apoptotic cells is shown. Data are from one experiment that was reproduced in independent experiments

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