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Fig. 3 | BMC Cancer

Fig. 3

From: MiR-193b promotes autophagy and non-apoptotic cell death in oesophageal cancer cells

Fig. 3

Evaluation of the consequences of Stathmin 1 knockdown on recovery of KYSE450 oesophageal cancer cells. a KYSE450 cells were transfected with stathmin 1 siRNA or scrambled siRNA (20 nM) and stathmin 1 levels were assessed by western blotting at 24, 48 and 72 h post-transfection. β-actin was used as a loading control. Stathmin 1 levels were normalised to actin and quantification is shown in the lower panel. Data is presented as mean +/- S.E.M (n = 2), (the 24 h time point is not a duplicate. However, 48 and 72 h transfection time points correspond to 24 and 48 h time points in the clonogenic assays below). b KYSE450 cells were transfected with stathmin 1 siRNA or scrambled siRNA (20 nM) and 24 h later were treated with 5-FU (10 μM or 30 μM) for 48 h. Drug-treated and control cells were counted and 1500 KYSE450 cells were re-seeded in triplicate wells without drug and allowed to grow for 12 days. Colonies were fixed and counted using the Odyssey Infrared Imaging System. Representative wells are shown. Asterisks indicate statistical significance determined by t-test (*p < 0.05, ***p < 0.001)

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