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Fig. 2 | BMC Cancer

Fig. 2

From: MiR-193b promotes autophagy and non-apoptotic cell death in oesophageal cancer cells

Fig. 2

Examination of the consequences of miR-193b overexpression on recovery of KYSE450 oesophageal cancer cells. a KYSE450 cells were transfected with miR-193b mimic or negative control mimic (5 nM) and were assessed for expression of stathmin 1 (miR-193b target) by western blotting at 48 and 72 h post-transfection. β-actin was used as a loading control. Stathmin 1 levels were normalised to actin and quantification is shown in the lower panel. Data is presented as mean +/- S.E.M (n = 2). KYSE450 cells were transfected with miR-193b mimic or negative control (5 nM) and 24 h later were treated with 5-FU (10 μM or 30 μM) for 24 (b) and 48 (c) hours. Drug-treated and control cells were counted and 1500 KYSE450 cells were re-seeded in triplicate wells without drug and allowed to grow for 12 days. Colonies were fixed and counted using the Odyssey Infrared Imaging System (Li-Cor). (C ii) The number of colonies observed for negative control mimic + 30 μM 5-FU and miR-193b mimic + 30 μM 5-FU treatments (boxed) were below the detection limit of the Odyssey Imaging system and were manually counted. Representative wells are shown. Asterisks indicate statistical significance determined by t-test (**p < 0.01, ***p < 0.001)

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