Table 1 Overview of medium compositions
From: Tumor slice culture system to assess drug response of primary breast cancer
Medium I [19] | Medium II [20] | Medium III | Medium IV |
|---|---|---|---|
- DMEM: HAM's F12 = 2:1 - FCS 2 % - Hydrocortisone 0.3 μg/ml - Insulin 4 μg/ml - Transferrin 4 μg/ml - 3,3´,5 Triiodothyronine 1 ng/ml - EGF 8 ng/ml - Cholera toxin 7 ng/ml - Adenine 0.2 mg/ml - Antibiotics | - RPMI-1640 - FCS 10 % - L-Glutamine 2 nM - Hydrocortisone 5 μg/ml - Insulin 5 μg/ml - Cholera toxin 50 ng/ml - EGF 10 ng/ml - Antibiotics | - RPMI-1640 - FCS 10 % - Antibiotics | - DMEM: HAM's F10 = 1:1 - FCS 10 % - Antibiotics |