Fig. 4
From: Tumor slice culture system to assess drug response of primary breast cancer
Assessment of drug response by aberrant nuclear morphology. a Nuclear morphology suggestive for tumor cell death included: Karyolysis: nuclear fading caused by dissolution of the chromatin, Pyknosis: irreversible condensation of the chromatin causing nuclei to shrink in size, Karyorrhexis: destructive fragmentation of a pyknotic nucleus, Apoptotic bodies: late stage apoptosis with fragmented nuclei. b An example of altered nuclear morphology (black arrows) observed in a single tumor after increasing dilution of FAC treatment. Dilution #3 was the lowest dilution at which altered nuclear morphology was observed in this tumor. Scale bar represents 50 μm