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Fig. 5 | BMC Cancer

Fig. 5

From: Prolonged pemetrexed pretreatment augments persistence of cisplatin-induced DNA damage and eliminates resistant lung cancer stem-like cells associated with EMT

Fig. 5

Therapy-resistant lung cancer cells featuring stem-like and EMT properties are most vulnerable to treatment #3. a Untreated A549 cells were seeded at low density and colony formation was subsequently analyzed by phase-contrast microscopy after 7 days. Colonies were clustered into three distinct types, see text for details. b Quantification of colony formation at day 17 of the recovery phase. Data shown are the mean values and standard deviations of three independent experiments. **, P < 0.01 and *, P < 0.05. c Analysis of gene expression by rtPCR. Data shown are the mean values and standard deviations of three independent experiments. *, P < 0.05

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