Fig. 4

Prolonged MTA pretreatment enhances cisplatin-induced accumulation of persistent DNA damage. Basal H2AX phosphorylation was set at 5 % in untreated controls and used for normalization among experiments as described in the material and methods section. Cell cycle phase-specific H2AX phosphorylation levels were determined as described in Figure S5. Data shown are the mean values and standard deviations of three experiments. *Percentage of total cell population (mean from three experiments). neg = γH2AX negative; pos = γH2AX positive