Fig. 2

Prolonged MTA pretreatment augments cisplatin-induced senescence in A549 cells. a Forward and side scatter analysis by flow cytometry (without reseeding) at day 10 (10d rec) and day 14 (14d rec) of the recovery phase. b Representative images of cells acquired by phase contrast-based microscopy at day 17 of the recovery phase (reseeded at day 10). Arrows indicate cells which stain positive for senescence associated β-galactosidase activity. c Quantification of senescent cells based on increased β-galactosidase activity b. Represented are three independent experiments and bars indicate means and standard deviations. ***, P < 0.001, **, P < 0.01 and *, P < 0.05