Fig. 4

CCL15 mediates the recruitment of macrophages in FTC. a Transwell assay of Thp-1 cell line by different treatment for 12 h. Bar = 10 μm. b Thp-1 cell numbers were calculated in each field under 400 magnifications. Both rCCL15 and condition medium of FTC cancer cell lines (FTC133-CM and WRO-CM) enhanced the recruitment of Thp-1 (*p < 0.05, **p < 0.01, compared with serum-free medium). Anti-CCL15 neutralizing antibody abrogated the recruitment by FTC133-CM or WRO-CM. (**p < 0.01, ***p < 0.001, compared with condition medium plus isotype-matched IgG). Statistical analysis was performed using a two-tailed Student’s t-test and the data was presented as mean ± SD (n = 16). Three biological replications were performed, with consistent results