Fig. 2

CCL15 is highly expressed in FTCs and CCR1 is induced by FTC133-CM. a qRT-PCR analysis of the CCL15 expression level in fresh tissues of FTC and FA tissues. Relative expression was normalized by the expression of ACTB. Data was presented as mean ± SE (n = 4). b, c, d qRT-PCR analysis of the CCR1 expression level in Thp-1 cell line. Thp-1 cells were treated with 30 ng/ml PMA or conditional media of FTC133 (FTC133-CM) for 24 h (b); Thp-1 cells were treated with increasing doses of rCCL15 for 24 h, and 50 μM IFN was used as a positive control (c); Thp-1 cells were treated with 50 μg/ml rCCL15 for increasing time periods (d). Relative expression was normalized by the expression of CCR1 in Thp-1 under mock treatment. ACTB was used as internal control and data were presented as mean ± SD (n = 3). Experiments were repeated three times, with consistent results