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Fig. 3 | BMC Cancer

Fig. 3

From: Regulation of the tumour suppressor PDCD4 by miR-499 and miR-21 in oropharyngeal cancers

Fig. 3

a Schematic representation of the PDCD4 constructs with and without the 3’UTR. b mRNA expression of PDCD4 in SCC089 cells transfected with PDCD4 constructs with and without the 3’UTR. These cells were also co-transfected with either miR-21, miR-499 alone or in combination and harvested 24 h post transfection. PDCD4 levels were only normalized to the reference gene B2M and fold change calculated using DeltaDelta Ct method. c Protein expression of PDCD4 in the co-transfected cell described in (B). PDCD4 expression was then quantitated relative to Beta-actin levels

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