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Fig. 8 | BMC Cancer

Fig. 8

From: Aberrant KDM5B expression promotes aggressive breast cancer through MALAT1 overexpression and downregulation of hsa-miR-448

Fig. 8

KDM5B is a functional target of hsa-miR-448 in TNBC cells. a KDM5B, MALAT1 and hsa-miR-448 protein or nucleotide input sequence obtained from the National Center for Biotechnology Information (NCBI) database, http://www.ncbi.nlm.nih.gov/. b Predicted KDM5B 3′-UTR binding site for hsa-miR-448. The hsa-miR-448 seed region alignment with KDM5B 3′-UTR is shown. c Upper panel: Sequence-based prediction of KDM5B-hsa-miR-448 interaction, with random forest, RF classifier score of 0.7 and support vehicle machine, SVM classifier score of 0.81. Lower panel: Sequence-based predicted interaction of KDM5B-MALAT1-hsa-miR-448, with RF classifier score of 0.75 and SVM classifier score of 0.997. * Interaction probabilities generated by RPISeq range from 0 to 1. In performance evaluation experiments, predictions with probabilities > 0.5 were considered “positive,” i.e., indicating that the corresponding RNA and protein are likely to interact. d The effect of KDM5B knockdown on the expression of KDM5B transcripts using qRT-PCR. e Loss of KDM5B function altered hsa-miR-448 mRNA expression in MDA-MB-231 cells. MDA-MB-231 cells were infected with scramble (231 V), shKDM5B clone II (231 II) or clone III (231 III) for 72 h. The transcript levels of KDM5B and hsa-miR-448 were assessed by qRT-PCR. f The mRNA expression of KDM5B and hsa-miR-448 is inversely correlated. Data are representative of 3 independent experiments and analyzed by student’s t-test. All data are shown as mean ± SEM. **p < 0.01, ***p < 0.001

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