Fig. 3

KDM5B enhances the proliferation and maintenance of TNBC cells malignant phenotype. a Proliferation assay showing that KDM5B knockdown inhibited cell proliferation, (b) silencing KDM5B suppressed the ability of the shKDM5B infected MDA-MB-231 cells to form mammospheres, and (c) colony formation assay showed that vector-infected cells possessed greater clonogenicity than their counterpart shKDM5B infected MDA-MB-231 cells. The experiments were carried out in triplicate. Scale bar: 200 μm. d Western blot showed that in human breast tissue samples KDM5B expression is higher in the TNBC tissues, compared to their non-TNBC counterparts and (e) the expression profile of KDM5B in different breast cancer cell lines, is similar to that of Survivin, c-Met, slug, c-Myc and N-cadherin, and converse to that in E-cadherin. GAPDH and a-tubulin were used as loading control. KDM5B, lysine specific demethylase 5B protein; 231, MDA-MB-231; 453, MDA-MB-453; **P < 0.01