Fig. 1

KDM5B expression is upregulated in human breast cancer tissues and cell lines. KDM5B is overexpressed in triple negative TGCA (a) invasive lobular breast cancer, and (b) invasive ductal breast cancer tissues, compared to their counterpart normal breast tissues. 1, normal breast tissue; 2, invasive breast carcinoma (c) Representative photomicrograph of immunohistochemical staining of KDM5B in human breast cancer tissues showed that KDM5B expression occurs preferentially in TNBC late tumor stage samples. Photographs were taken at 200× magnification. Western blot showing KDM5B expression in (d, e) paired tumor: non-tumor human breast samples. f Western blot analysis of KDM5B expression in 7 different breast cancer cell lines. KDM5B is strongly expressed in TNBC cell lines, MDA-MB-231, MDA-MB-453 and HS-578 T. GAPDH and α-tubulin were used as loading control. Experiments were done in triplicate and quantified by ImageJ densitometry. KDM5B, lysine specific demethylase 5B protein; T, tumor; NT, non-tumor; DCIS, ductal carcinoma in situ; 231, MDA-MB-231; 453, MDA-MB-453