Fig. 5
Expression of selected mRNAs determined by qPCR in RKO and DLD-1 cell derivatives. a. Immunoblot analysis of total cell extracts from DLD-1 derivatives stably transfected with empty vector (DLD-1CTRL) or with transgelin cDNA (DLD-1TAGLN). b. Real-time RT-PCR analysis for the levels of mRNA in RKO and DLD-1 cells of selected top-ranked differentially expressed genes identified by the cDNA microarray. Gene expression was normalized to GAPDH. Error bars denote standard deviation. All changes were significant (P < 0.05 by Student’s t-test) except for the one result indicated (#)