Fig. 2From: Transgelin increases metastatic potential of colorectal cancer cells in vivo and alters expression of genes involved in cell motilityEffects of TAGLN overexpression on in vitro cell behavior. a. Invasiveness. Left, representative images showing invasion of RKOTAGLN and RKOCTRL cells through Matrigel-coated Transwell filters, right, quantification of filter staining. b. Clonogenicity. Left, representative images of plates seeded with RKOTAGLN or RKOCTRL cells, right, quantification of colony formation after 12 days. c. Growth in soft agar. Left, representative images of colonies formed by RKOTAGLN and RKOCTRL cells, right, quantification colony formation after 17 days. d. Cell proliferation. Graph shows cell count in replicate cultures of RKOTAGLN and RKOCTRL, counted daily for four days. Graphs in panels a-d show mean of three experiments. Error bars denote standard deviation. e. Cell cycle distribution. Graph shows the percentage of RKOTAGLN and RKOCTRL cells in G0/G1, S, and G2/M phases of the cell cycle. Data are mean of technical replicates from a single representative experiment. Error bars denote standard deviation. ** P < 0.01 by Student’s t-testBack to article page