| |
C
|
W
|
A
|
L
|
LW
|
LA
|
Interaction effectb
|
Tumour effectb
|
Diet effectb
|
|---|
|
Foetal resorption per dam
|
0.900±0.314
|
3.300±0.538*
|
2.500±0.562*
|
0.889±0.309
|
1.600±0.499
|
1.700±0.473
|
P = 0.0321
|
P = 0.0043
|
n.s.
|
|
Foetal weight (g)
|
3.92 ± 0.07
|
2.73 ± 0.20*
|
2.61 ± 0.05*
|
3.65 ± 0.06
|
2.85 ± 0.08*
|
2.96 ± 0.09*
|
n.s.
|
P < 0.0001
|
n.s.
|
|
Placenta weight (g)
|
0.48 ± 0.01
|
0.53 ± 0.02
|
0.42 ± 0.02
|
0.52 ± 0.03
|
0.52 ± 0.01
|
0.50 ± 0.02
|
n.s.
|
n.s.
|
n.s.
|
|
Placental protein content (μg/mg)
|
7.38 ± 0.48
|
3.81 ± 0.29*
|
4.70 ± 0.34*
|
7.14 ± 0.28
|
6.87 ± 0.56
|
6.37 ± 0.67
|
P = 0.0043
|
P = 0.0002
|
n.s.
|
|
Placental DNA (μg/mg)
|
0.46 ± 0.02
|
0.38 ± 0.02*
|
0.38 ± 0.02*
|
0.46 ± 0.03
|
0.49 ± 0.02
|
0.43 ± 0.04
|
n.s.
|
P = 0.0052
|
n.s.
|
|
Placental protein/DNA ratioa
|
16.8 ± 1.2
|
11.5 ± 0.9*
|
14.2 ± 1.5
|
17.3 ± 1.1
|
15.4 ± 1.5
|
17.3 ± 2.5
|
P = 0.0212
|
P = 0.0311
|
n.s.
|
- Caption: Control (C), Leucine (L), Walker tumour-bearing rats (W), tumour-bearing rats subjected to a leucine-rich diet (LW), rats inoculated with ascitic fluid (A), and rats inoculated with ascitic fluid and subjected to a leucine-rich (LA). The results are expressed as the mean and standard deviation. *P < 0.05 compared with the control group. aPlacental protein/DNA ratio was used as an index of cell size. b Significant difference accounted by tumour, diet or interaction effect, analysed by two-way ANOVA with Bonferroni testing, n.s. = non-significant
