Table 1 Detailed primers used to access the protein degradation and synthesis processes in placenta tissue
Protein degradation -associated | |||
Gene | Forward | Reverse | |
PC5 | NM_053590.1 | GGACTTGATGAAGAAGGAAAGG | GATGCCCTCTTTGGTCACTATG |
PC2 | NM_017278.1 | CAGGAGTGTTTGGATTCCAG | GTTAGCAGATGGACAGGTTTGG |
Murf-1 | NM_080903 | TGAAGTGATCATGGACCGGC | AATGCTCTTGATGAGCGGCTT |
Atrogin | NM_133521 | ATGCCGTTCCTTGGTCAGG | ATGGCGCTCCTTAGTACTCCC |
Ubiquitin | NM_031138.2 | GAGGCTCATGCGGGATTTCAAG | TCCTGATAAAGCTGTGCTGC |
Calpain | AB384822.1 | GAACCGCATCCGGAATTACCTG | GGTTGCAGCTGACCATGTTTGCA |
Protein synthesis-associated genes | |||
mTOR | NM_001134499.2 | CTCGTGAAGGACAACGGTCA | TAGAGTTTTTCGTGGGCGCT |
p70S6K1 | NM_031985 | GGCCCTGGGGATGCTGGAGA | GGCTGACAGGCGTTCGTGGG |
eIF4E | NM_053974 | ACCCCTACCACTAATCCCCC | GCTTCAGTGCAGTCCACTCT |
eIF4G | NM_013507.3 | ACCCACAATGGGACGTCATC | CCGAGGTGGCATATCCTTCG |
4EBP1 | NM_053857.2 | CGGCACGCTCTTCAGCACCA | ACACCCAGTGTCTGCCGGGT |
eIF5 | XM_001479599.1 | TGTCTGTCAACGTCAACCGCAG | GAGGCATGCTTGACACACATC |
eIF2α | AK312442.1 | GATCCATTGCTGAAATGGGG | GATGACAAGTACAAGAGACCTGG |