Fig. 6

Key proteins involved in proteolysis in the placental tissues of pregnant, Walker tumour-bearing rats and ascitic fluid-injected rats fed either a control or leucine-rich diet. Expression levels of ubiquitin-proteasome pathway members, including the 19S (a), 20S (b, c, and d) and 11S subunits; (e), muscle-ring finger protein 1 (MuRF-1) (f); and the eukaryotic initiation factor eIF2a (g) in placenta tissue was quantified by western blotting. For additional details, see the Materials and Methods section. The results are expressed as the mean and standard error. The images represent one of 6 animals per group. Legend: Control (C), Leucine (L), Walker tumour-bearing rats (W), tumour-bearing rats fed a leucine-rich diet (WL), ascitic fluid-injected rats (A) and ascitic fluid-injected rats fed a leucine-rich diet (LA). * P < 0.05 accounted by tumour effect, two-way ANOVA with Bonferroni testing, n = 10. ϕ P < 0.05 accounted by diet effect, two-way ANOVA with Bonferroni testing, n = 10