Fig. 3

IHC analysis of the expression of E-cadherin, VCAM-1, ICAM-1 and CK8/18 (X 200). CK8/18 expression was detected in the surgical specimen used for implantation (a) and in the primary implanted tumor tissues (b), but not in the F1 generation implanted tumor tissues (c), VCAM-1 was expressed in the surgical specimen (d), and in the primary implanted tumor tissues (e), but not in the F2 generation implanted tumor tissues (f). E-cadherin expression was not detectable in the surgical specimen (g) and in the primary implanted tumor tissues (h). ICAM-1 was expressed in the surgical specimen (i), but not in the primary implanted tumor tissues (j)