Fig. 7

a In vivo tumor formation assay using PFL treated or non-treated MKN28-EGFP cells. MKN28-EGFP cells were incubated with 10 μM PFL for 3 h and injected to the portal vein of mice. Tumor colonies formed in the liver were detected 4 weeks post-injection. Photographs show the dissected livers (upper panels). Tumor colonies are indicated by white arrows for control (left) and PFL-treated groups (right). Representative IVIS images are shown at the bottom. b In vivo effect of PFL on tumor growth in nude mice. MKN28 cells were subcutaneously injected to mice and tumors were allowed to grow. PFL (100 μg) in 100 μL PBS was intratumorally-injected three times 1, 3, and 7 days following tumor cells implantation. The histogram displays tumor volume, represented as means ± SD (n = 10). Asterisks indicate values significantly different (p < 0.01) between PFL-treated mice and the control. Representative IVIS images are shown for PFL-treated mice and the control