Fig. 2

a Identification of a cell surface molecule that interacts with PFL on MKN28 cells. The cells were treated with biotin-PFL and proteins that bound to biotin-PFL were precipitated with avidin-coated beads. Proteins captured on beads were separated by SDS-PAGE (left panel) and the protein specific for the PFL fraction (arrow 1) was analyzed by MALDI-TOF MS after in-gel digestion with trypsin. The peptide mass finger printing data (right panel) thus obtained was searched using Mascot software. b Effect of PFL on cellular distribution of EGFR. The change of cellular localization of EGFR induced by PFL was observed using confocal fluorescence microscopy. The distribution of Alexa-488 PFL (green) and EGFR (red) are shown. Colocalization of Alexa-488 PFL and EGFR is shown as a yellow signal (Merge). Nuclei within the cells were stained with DAPI. In the right panel, the effect of yeast mannan (YM) on PFL-induced trafficking of EGFR is shown. The change in distribution of EGFR (green) was observed by confocal fluorescence microscopy. c Western blots show the protein levels of EGFR in MKN28 cells upon treatment with 2 μM PFL for 3, 24, 48, and 72 h and without treatment (shown as -). In the right panel, western blots show the protein levels of EGFR in MKN28 cells determined in the presence or absence of 2 μM PFL