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Fig. 1 | BMC Cancer

Fig. 1

From: High mannose-binding Pseudomonas fluorescens lectin (PFL) downregulates cell surface integrin/EGFR and induces autophagy in gastric cancer cells

Fig. 1

a Change of adhesive properties of cells to ECM after PFL treatment. MKN28 cells were treated with 10 μM PFL for 3 h and spread on ECM-coated plates. The cells were allowed to attach to ECM-coated plates for 90 min at 37 °C. Attached cells were detected by a colorimetric method. Controls were evaluated in the same way without PFL. b Down regulation of surface α2 integrin in MKN28 cells after a 24 h incubation with 10 μM PFL. Cellular distribution of α2 integrin after PFL treatment was observed by immunofluorescence microscopy using anti- α2 integrin antibody

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