Table 1 Specific primers used in qPCR
Gene | Forward primer (5’-3’) | Reverse Primer (5’-3’) |
|---|---|---|
AFP | AGACTGAAAACCCTCTTGAATGC | GTCCTCACTGAGTTGGCAACA |
CD133 | ACATGAAAAG ACCTGGGGG | GATCTGGTGTCCCAGCATG |
CD44 | TCCCAGACGAAGACAGTCCCTGGAT | CACTGGGGTGGAATGTGTCTTGGTC |
ALDH1A1 | CTGCTGGCGACAATGGAGT | CTGCTGGCGACAATGGAGT |
ABCB1 | GGGAGCTTAACACCCGACTTA | GCCAAAATCACAAGGGTTAGCTT |
EpCAM | AATCGTCAATGCCAGTGTACTT | TCTCATCGCAGTCAGGATCATAA |
Bcl-2 | GACTGAATCGGAGATGGAGACC | GCAGTTCAAACTCGTCGCCT |
β-actin | CATCCTCACCCTGAAGTACCC | AGCCTGGATAGCAACGTACATG |