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Table 1 Primer sequences used in the study

From: Cooperation of Sox4 with β-catenin/p300 complex in transcriptional regulation of the Slug gene during divergent sarcomatous differentiation in uterine carcinosarcoma

Assay Gene/region   Sequence Size
Mutagenesis Slug/Sox4-M1 Forward 5′-ACTTTTAGGGGTTGTGGATAGACTGTGT-3′  
   Reverse 5′-ACAACCCCTAAAAGTGTTAGACAATGT-3′  
  Slug/Sox4-M2 Forward 5′-AGGATTAGGGTGAATTATTTTCTCTGTT-3′  
   Reverse 5′-AATTCACCCTAATCCTTATGCTAATGGA-3′  
  Slug/Sox4-M3 Forward 5′-AATAATAGGGGAAATTAGCTTAGGAAAT-3′  
   Reverse 5′-ATTTCCCCTATTATTCTTATTTCTTCC-3′  
  Slug/Sox4-M4 Forward 5′-GAGGGCAGGGAAGCATTTCTTTCAAGCC-3′  
   Reverse 5′-TGCTTCCCTGCCCTCTAAAGGCAGGCT-3′  
ChIP Slug/Sox4-1 Forward 5′-GTGTTATAACTACCAGCAAA-3′ 132 bp
   Reverse 5′-ACAAATATAGCACAGTTGAG-3′  
  Slug/Sox4-2 Forward 5′-TCTCCTGCAAGTACAGTTCC-3′ 149 bp
   Reverse 5′-TGTTTGGAGGGTGAGGTGG-3′  
  Slug/Sox4-3 Forward 5′-AGTGACTGTTGGAAGAAATA-3′ 141 bp
   Reverse 5′-AAAGTGCATTGTCAGGTTG-3′  
  Slug/Sox4-4 Forward 5′-TCAGCCTGCCTTTAGAGGGC-3′ 121 bp
   Reverse 5′-GCTACTCAGGGCTTCCGCG-3′  
mRNA Slug Forward 5′-ACGCAATCAATGTTTACTCG-3′ 277 bp
   Reverse 5′-TGAAGAGAAAGGTTACTGTC-3′  
  E-Cadherin Forward 5′-CAACATGGGAGGTGAGAGTTT-3′ 319 bp
   Reverse 5′-CGAAGAAACAGCAAGAGCAGCAGAATCAGA-3′  
  Sox4 Forward 5′-GTTCGGCGTGTGCTTGGC-3′ 261 bp
   Reverse 5′-GTCTTGCACCAGCTCGGG-3′  
  Sox7 Forward 5′-AAGCCCTCTCCACTGTAGCC-3′ 245 bp
   Reverse 5′-TTGCGATCCATGTCCCCCAG-3′  
  Sox9 Forward 5′-CAGCAAGAACAAGCCGCACG-3′ 222 bp
   Reverse 5′-GTAATCCGGGTGGTCCTTCTT-3′