Table 1 Primer sequences used in the study
Assay | Gene/region | Sequence | Size | |
|---|---|---|---|---|
Mutagenesis | Slug/Sox4-M1 | Forward | 5′-ACTTTTAGGGGTTGTGGATAGACTGTGT-3′ | |
Reverse | 5′-ACAACCCCTAAAAGTGTTAGACAATGT-3′ | |||
Slug/Sox4-M2 | Forward | 5′-AGGATTAGGGTGAATTATTTTCTCTGTT-3′ | ||
Reverse | 5′-AATTCACCCTAATCCTTATGCTAATGGA-3′ | |||
Slug/Sox4-M3 | Forward | 5′-AATAATAGGGGAAATTAGCTTAGGAAAT-3′ | ||
Reverse | 5′-ATTTCCCCTATTATTCTTATTTCTTCC-3′ | |||
Slug/Sox4-M4 | Forward | 5′-GAGGGCAGGGAAGCATTTCTTTCAAGCC-3′ | ||
Reverse | 5′-TGCTTCCCTGCCCTCTAAAGGCAGGCT-3′ | |||
ChIP | Slug/Sox4-1 | Forward | 5′-GTGTTATAACTACCAGCAAA-3′ | 132 bp |
Reverse | 5′-ACAAATATAGCACAGTTGAG-3′ | |||
Slug/Sox4-2 | Forward | 5′-TCTCCTGCAAGTACAGTTCC-3′ | 149 bp | |
Reverse | 5′-TGTTTGGAGGGTGAGGTGG-3′ | |||
Slug/Sox4-3 | Forward | 5′-AGTGACTGTTGGAAGAAATA-3′ | 141 bp | |
Reverse | 5′-AAAGTGCATTGTCAGGTTG-3′ | |||
Slug/Sox4-4 | Forward | 5′-TCAGCCTGCCTTTAGAGGGC-3′ | 121 bp | |
Reverse | 5′-GCTACTCAGGGCTTCCGCG-3′ | |||
mRNA | Slug | Forward | 5′-ACGCAATCAATGTTTACTCG-3′ | 277 bp |
Reverse | 5′-TGAAGAGAAAGGTTACTGTC-3′ | |||
E-Cadherin | Forward | 5′-CAACATGGGAGGTGAGAGTTT-3′ | 319 bp | |
Reverse | 5′-CGAAGAAACAGCAAGAGCAGCAGAATCAGA-3′ | |||
Sox4 | Forward | 5′-GTTCGGCGTGTGCTTGGC-3′ | 261 bp | |
Reverse | 5′-GTCTTGCACCAGCTCGGG-3′ | |||
Sox7 | Forward | 5′-AAGCCCTCTCCACTGTAGCC-3′ | 245 bp | |
Reverse | 5′-TTGCGATCCATGTCCCCCAG-3′ | |||
Sox9 | Forward | 5′-CAGCAAGAACAAGCCGCACG-3′ | 222 bp | |
Reverse | 5′-GTAATCCGGGTGGTCCTTCTT-3′ |