Fig. 4

Interaction among β-catenin, Sox4, and p300 in Em Ca cells. a Ishikawa cells were transfected with Slug reporter constructs, together with β-cateninΔS45 (β-cat), Sox4, p300, and dominant-negative TCF4 (ΔNTCF4). Relative activity was determined based on arbitrary light units of luciferase activity normalized to pRL-TK activity. The activities of the reporter plus the effector relative to that of the reporter plus empty vector are shown as means ± SDs. The experiment was performed in duplicate. b After transfection of HA-β-cateninΔS45, GFP-Sox4, and Flag-p300, Ishikawa cell were stained with anti-HA antibody (upper) or a combination of anti-HA and anti-Flag antibodies (lower). Immunopositive cells are indicated by arrows. Nuclei were stained with DAPI. c Ishikawa cells were transfected with pM-β-catenin (left) or pM-Sox4 (right), along with pGL5 luc, Sox4, Sox7, Sox9, and p300. The experiment was performed in duplicate. d Coimmunoprecipitation of β-catenin and Sox4 in Ishikawa, Hec251, and Hec6 cells cultured in STK2 for 5 days. IP, immunoprecipitation