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Fig. 1 | BMC Cancer

Fig. 1

From: Cooperation of Sox4 with β-catenin/p300 complex in transcriptional regulation of the Slug gene during divergent sarcomatous differentiation in uterine carcinosarcoma

Fig. 1

Changes in expression of Sox factors during the EMT process in Em Ca cells. a Phase-contrast images of Ishikawa, Hec251, and Hec6 cells cultured in STK2 for 5 days. Western blot analysis (b) and real time RT-PCR (c) for the indicated molecules from Ishikawa, Hec251, and Hec6 cells cultured in STK2. Con and c, control; s, STK2; Ish, Ishikawa cells. d Western blot analysis for the indicated proteins from Ishikawa, Hec251, and Hec6 cells cultured in STK2. Con, control. e Real time RT-PCR for the indicated mRNA transcripts from Ishikawa, Hec251, and Hec6 cells cultured in STK2. c, control; s, STK2. f Hec6 cells were transfected with Sox4, Sox7, and Sox9 reporter constructs, together with either Sox4, Sox7, or Sox9. Relative activity was determined based on arbitrary light units of luciferase activity normalized to pRL-TK activity. The activities of the reporter plus the effector relative to that of the reporter plus empty vector are shown as means ± SDs. The experiment was performed in duplicate

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