Table 1 Primers used for MSP
From: Gene methylation profile of gastric cancerous tissue according to tumor site in the stomach
Primer name | Forward sequence | Reverse sequence | Reference number |
|---|---|---|---|
MLH1 methylated | TATATCGTTCGTAGTATTC [GenBank:NG_0071092] | TCCGACCCGAATAAACC | [40] |
MLH1 unmethylated | TTTTGATGTAGATGTTTTATTAGGGT [GenBank:NG_0071092] | TACCACCTCATCATAACTACC | [40] |
MGMT methylated | TTTCGACGTTCGTAGGTTTTCGC [GenBank:NG_000010.11] | GCACTCTTCCGAAAACGAAAC G | [40] |
MGMT unmethylated | TTTGTGTTTTGATGTTTGTAGGTTTTTGT [GenBank:NG_000010.11] | AACTCCACACTCTTCCAAAAACAAAACA | [40] |
DAPK-1 methylated | GGATAGTCGGATCGAGTTAACGTC [GenBank:NG_029883.1] | CCCTCCCAAACGCCG A | [40] |
DAPK-1 unmethylated | GGAGGATAGTTGGATTGAGTTAATGTT [GenBank:NG_029883.1] | CAAATCCCTCCCAAACACCAA | [40] |