Fig. 4

Effects of TLN-1/2 knockdown on migration and invasion in MHCC-97 L cells. a Migration and (b) invasion abilities of MHCC-97 L cells were examined by transwell assays. The trans-membrane cells were fixed in 4 % paraformaldehyde and dyed using crystal violet (magnification: ×200). Quantification of migrating or invading cells was determined using the MTT assay. OD: Optical density. c Migrating ability of MHCC-97 L cells was also determined by wound healing assay at 0 and 72 h after scratching. The tissue monolayer was imaged under light microscopy (magnification: ×200). Data are mean ± SD. **P < 0.01 vs. Blank; ^##P < 0.01 vs. scramble shRNA