Fig. 2

Effects of TLN-1 knockdown on TLN-1 and TLN-2 mRNA and protein expression in MHCC-97 L cells. a TLN-1 and (b) TLN-2 mRNA expression levels were assessed by real-time RT-PCR at 12 h, 48 h and 72 h after transduction. GAPDH was used as an internal control. c TLN-1 and (d) TLN-2 protein amounts were determined by Western blot at 12 h, 48 h and 72 h after transduction. β-actin was used as an internal control. Data are mean ± SD. **P < 0.01 vs. Blank; ^##P < 0.01 vs. scramble shRNA