Fig. 4

Intracellular iron concentration is depleted by both deferasirox and irradiation in U251 cells but only under normoxic condition. Intracellular iron concentration in U251 glioblastoma cells cultivated at 21 % (a) or 3 % (b) of oxygen and in U87 glioblastoma cells cultivated at 21 % (c) or 3 % (d) of oxygen in untreated condition (CONT) or 3 days after treatment with 10 μM of deferasirox (DFX) in non-irradiated condition (0 Gy) or following irradiation with 16 Gy (16 Gy). Intracellular iron concentration is expressed in nmol per mg of protein as mean ± standard deviation (SD) (n = 3). One-way ANOVA was performed between DFX treatment and CONT in non-irradiated (0 Gy) or irradiated (16 Gy) conditions (^*, p-value ≤0.05; ^***, p-value ≤0.001). Two-way ANOVA was performed between non-irradiated (0 Gy) condition and irradiated (16 Gy) condition (^$$$, p-value ≤0.001)