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Fig. 3 | BMC Cancer

Fig. 3

From: Mutations in TP53 increase the risk of SOX2 copy number alterations and silencing of TP53 reduces SOX2 expression in non-small cell lung cancer

Fig. 3

a Efficient SOX2 and TP53 knockdown was confirmed by western blotting. SOX2 and TP53 were silenced using siRNA technology. After 48 h nuclear lysates were isolated from knockdown and control cells. 40 µg lysate/lane was resolved in a SDS-PAGE and membrane analysed for expression of SOX2, TP53 and GAPDH (loading control) by western blotting. b Protein expression was semiquantitatively analysed by densitometry using Image J. Bars indicate mean ± SE, n = 3. ***indicating p < 0.001 and **indicating p < 0.01 was obtained from one-way ANOVA

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