Fig. 6

Expression analysis of hnRNPA1, HP1α-V3 and STET in breast cancer biopsies. a-e Based on a standard curve of serial dilutions of cDNA with known concentrations, quantification was determined from single measurements with the second derivate max method by the LightCycler software. Relative expression was calculated using HMBS for normalization. Results are presented as log-transformed values of HMBS normalized data. N indicates the number of samples with measurements above limit of detection. The bars represent the mean value with surrounding standard deviations. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. One-way ANOVA with Tukey’s multiple comparisons. For datasets with significantly different standard deviations between means (HP1α-V3/hnRNPA1 and HP1α-V3/STET), non-parametric Kruskal-Wallis tests with Dunn’s multiple comparisons were performed