Fig. 3

TSA is a negative regulator of the CBX5 and hnRNPA1 locus. a TSA mediates CBX5 down-regulation. Relative expression of CBX5 derived transcripts and hnRNPA1 in MCF7 and MDA-MB-231 cells in a TSA response after 24 h treatment. Relative expression was calculated from RT-qPCR using GAPDH expression for normalization. CBX5 transcript primers were specific for the indicated isoforms and HP1α-pan was detected by an exon 4 and 5 primer combination. b TSA down-regulates HP1α expression at the protein level. Protein extracts were isolated from MCF7 and MDA-MB-231 cells in a TSA response after 24 h treatment. Western blot analyses were performed with antibodies for HP1α and β − Actin for loading control using the same membrane. c H3-K9ac and H3 ChIP analysis of the CBX5-hnRNPA1 locus. ChIP analysis were performed from MDA-MB-231 control cells or treated with TSA for 24 h. The presented data shows the H3K9ac signal relative to H3 signal. Positions of PCR primers are indicated. The control region is located in the un-transcribed genomic position. For all panels, bars represent mean values with standard deviations