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Fig. 6 | BMC Cancer

Fig. 6

From: A potent betulinic acid analogue ascertains an antagonistic mechanism between autophagy and proteasomal degradation pathway in HT-29 cells

Fig. 6

Effect of autophagy inhibitor 3-MA in 2c induced cytotoxicity. HT-29 cells (2.5 × 104/100 μl of RPMI 1640 medium / well) were pre-treated with autophagy inhibitor, 3-MA (10 mM) for 4 h followed by 2c treatment for 48 h. Cell viability was measured by MTT assay. Histograms represent percentage cell viability (Mean ± SEM) obtained at the IC50 concentration of 2c (14.9 μM) and has been derived from at least three experiments in duplicate (***p <0.001 compared to only 2c treated cells)

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