Fig. 13From: A potent betulinic acid analogue ascertains an antagonistic mechanism between autophagy and proteasomal degradation pathway in HT-29 cellsEffect of 3-MA and Z-VAD-FMK on apoptosis and autophagy. HT-29 cells were incubated with IC50 concentration of 2c (48Â h) after treatment with 3-MA (10Â mM; 4Â h) or Z-VAD-FMK (20Â mM; 4Â h) as well as untreated cells also. (a) Cell viability was measured after Z-VAD-FMK (20 mM, 4 h) treatment. (b) Whole cell lysates were prepared and subjected to immunoblot analysis using specific antibodies against Bax or LC3. Analysis was confirmed with three different sets of experimentsBack to article page