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Fig. 12 | BMC Cancer

Fig. 12

From: A potent betulinic acid analogue ascertains an antagonistic mechanism between autophagy and proteasomal degradation pathway in HT-29 cells

Fig. 12

Co-localization of proteins associated with autophagy. Immunofluorescence (IF) demonstrates (a) colocalization of LC3B (green) with Lysosome (red), p62 (green) with Lysosome (red), Ubiquitin (green) with Lysosome (red), LAMP-1(green) with LC3B (red) and p62 (green) with LC3B (red) in HT-29 cells without 2c treatment. b colocalization of LC3B (green) with Lysosome (red), p62 (green) with Lysosome (red), Ubiquitin (green) with Lysosome (red), LAMP-1(green) with LC3B (red) and (c) p62 (green) with LC3B (red) counterstained with DAPI (blue) in HT-29 cells treated with 2c for 24 h. d percentage of cellular puncta co-localized for five pair of protein sets were determined. Scale bars, 10 mm. The bars represent Mean ± SEM

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