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Fig. 6 | BMC Cancer

Fig. 6

From: CD133+ liver cancer stem cells resist interferon-gamma-induced autophagy

Fig. 6

IFN-γ increased the autophagy of BEL7402 and QGY7701. a LC3 distribution in four cell lines treated with or without 10 ng/ml IFN-γ for four days detected by confocal. b Left, immunoblotting results of LC3 and p62 expression in four cell lines treated with or without 10 ng/ml IFN-γ for four days. Right, expression of indicated molecular were semi-quantitative analyzed with imageJ. 8024, PLC8024; 7402, BEL7402; 7701, QGY7701. C, control; IFN, IFN-γ. *, p < 0.05. c immunoblotting results of LC3 expression in BEL7402 and QGY7701 treated with or without 10 ng/ml IFN-γ for four days after Atg5 siRNA knockdown. d proliferation assay of BEL7402 and QGY7701 cells treated with 10 ng/ml IFN-γ after Atg5 knockdown detected by CCK-8 assay. si-1, siAtg5-1; si-2, siAtg5-2. *, p < 0.05. Representative of three independent experiments

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