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Fig. 2 | BMC Cancer

Fig. 2

From: RASSF1 tumor suppressor gene in pancreatic ductal adenocarcinoma: correlation of expression, chromosomal status and epigenetic changes

Fig. 2

Representative FISH digitalized images of the RASSF1 and chromosome 3 status in PDAC. The DNA probes used were a home-made Spectrum Orange for the locus specific RASSF1 gene and a commercially available Spectrum Green enumeration CEP3 probe: a diploid status for chromosome 3 and RASSF1 loci; b gains of chromosome 3 (four signals) and two RASSF1 signals reflecting loss in a chromosome 3 tetraploid nucleus; c loss of RASSF1 locus in two diploid chromosome 3 nuclei. The digitalized images are obtained by the High Technology Scan D-Sight/Fluo software (Visia Imaging, San Giovanni Valdarno, Italy), which also recognizes and circles individual neoplastic DAPI stained nuclei

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