Fig. 6

Western blot analysis of prostasin and matriptase expression in UROtsa and human bladder transitional cell carcinoma cell lines (UC-9 and UC-5). The UROtsa cells were treated with 2 mM ibuprofen and cancer cells were treated with 0.5 mM ibuprofen for 24 h. Twenty-five micrograms of total protein from each sample were loaded in each lane. Samples from two separated experiments were shown. The numbers under each lane indicated the fold change of the expression as compared to the DMSO control. IBU: ibuprofen. GAPDH was used as a loading control