Fig. 5

HGS knockdown impairs cell viability specifically in HCT116 cells with oncogenic mutations in β-catenin. HCT116^wt/del (CTNNB1^S45del heterozygous) and HCT116^WT/- (CTNNB1^WT only) cells were transfected with scramble or siRNA targeting specifically HGS and cultivated for 48 h. Untransfected cells were also included as a control. a HGS mRNA levels were assessed by RT-qPCR and normalized to the abundance of 18S RNA. The mean ± SD of triplicate samples is presented. b The abundance of HGS and γ-tubulin protein was examined by immunobloting. c Cell proliferation was visualized by crystal violet staining 72 h after HGS knockdown. Dye was solubilized in 1 % SDS solution and absorbance was read at 570 nm and quantified. The mean ± SD of triplicate samples is presented. d Assessment of apoptosis with DiOC6₍₃₎ and IP staining. The mean ± SD of three independent experiments is presented