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Table 2 Optimal number of reference miRNAs – pancreatic cancer

From: MicroRNA Expression in Formalin-fixed Paraffin-embedded Cancer Tissue: Identifying Reference MicroRNAs and Variability

Number of miRNAs Va ΔVa miRNAs added Stability increased?
2 miR-103a-3p, miR-374b-5p
3 vs 2 0.1884 miR-361-5p
4 vs 3 0.1328 -0.0556 let-7 g-5p yes
5 vs 4 0.1141 -0.0186 miR-28-5p yes
6 vs 5 0.0962 -0.0179 miR-29a-3p yes
7 vs 6 0.0831 -0.0131 miR-301a-3p yes
8 vs 7 0.0631 -0.0200 miR-340-5p yes
9 vs 8 0.0596 -0.0036 miR-27a-3p yes
10 vs 9 0.0522 -0.0073 miR-106b-5p yes
11 vs 10 0.0527 0.0005 miR-152-3p no
12 vs 11 0.0549 0.0021 miR-26b-5p no
13 vs 12 0.0464 -0.0084 miR-660-5p yes
14 vs 13 0.0470 0.0006 miR-24-3p no
15 vs 14 0.0517 0.0047 miR-23b-3p no
16 vs 15 0.0569 0.0052 miR-199a-3p no
  1. MiRNAs in bold are the ones that are included in the optimal number of references to use.
  2. a The stability measure V is calculated according to Vandesompele et al. [21]
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