Proapoptotic effect of THC is mediated via CB1 and CB2. a The CB1 antagonist LY320135 and a selective CB2 inverse ligand agonist (JTE-907) were tested in dose-dependent dilution series. Dose-effect plots from an apoptosis annexin V-based flow cytometry assay are shown. b MOLM13 and Jurkat cells were pretreated with either antagonist (LY, LY320135; JTE, JTE-907) for 12 h and THC was administered for another 48 h (30 μM for Jurkat and 45 μM for MOLM13 cells). Induction of apoptosis was analyzed as described above. (*-****) statistical significance at p < 0.05 (Student’s t-test). c Western immunoblotting of cleaved caspase 3 in response to THC +/- preexposition to LY320135 or JTE-907 is shown.