Fig. 2
Flow cytometric apoptosis assay measuring early apoptotic (annexin V) and later phase apoptotic cells (propidium iodide) after exposure of Jurkat (a-c) or MOLM13 (d-f) cells to THC. Dose-effect curves for Jurkat (a) and MOLM13 (d) cells treated with THC in a dose-dependent manner are shown. Student’s t-test demonstrates significance (p < 0.05) of induction of apoptosis at 46 μM (Jurkat), resp. 32 μM (MOLM13). Experiments were performed in triplicates. Non-linear regression analysis was performed to compute IC50s (b, e). Flow cytometry raw data are shown for Jurkat (c) and MOLM13 (f) cells demonstrating overwhelming induction of apoptosis in the highest tested doses – with no effect for methanol as drug carrier at the highest tested dose