Fig. 8
Effect of mAR activation on intracellular Ca2+ release and store operated Ca2+ entry (SOCE) in MCF-7 cells. a Representative tracings of fura-2 fluorescence-ratio in fluorescence spectrometry before, during and after Ca2+ depletion with subsequent addition of thapsigargin (1 μM) in MCF-7 cells without (control, open squares) and with (grey and black squares) treatment with testosterone-albumin-conjugates (TAC, 100 nM) for 15–120 min in the absence and presence of the Orai-1 inhibitor 2-APB (50 μM). b, c Arithmetic means (± SEM, n = 3–5, each experiment 10–30 cells) of slope (b) and peak (c) increase of fura-2-fluorescence-ratio following re-addition of extracellular Ca2+ in MCF-7 cells without (control, white bars) and with (grey and black bars) treatment with TAC (100 nM) for 15–120 min in the absence and presence of the Orai-1 inhibitor 2-APB (50 μM). ***(p < 0.001) indicates statistically significant difference from absence of TAC, ###(p < 0.001) indicates statistically significant difference from 60 min presence of TAC without presence of 2-APB (ANOVA)