Fig. 5

Effect of mAR activation on actin cytoskeleton and membrane Orai1 abundance of MCF-7 cells. a Original confocal microscopy of actin filaments (red) and Orai1 (green) in non-permeabilized MCF-7 cells without (control) and with a prior 60 min treatment with testosterone-albumin-conjugates (TAC, 100 nM) alone (TAC) or together with cytochalasin B (1 μM) (TAC + cytochalasin B), or with Rac inhibitor NSC23766 (50 μM) (TAC+ Rac inhibitor). b, c Arithmetic means ± SEM of (b) Orai1 abundance (n = 6) and of (c) actin fluorescence (n = 6) in MCF-7 cells without (white bar) and with (black bars) a 60 min treatment with testosterone-albumin-conjugates (TAC, 100 nM). ***(p < 0.001) indicates statistically significant difference from absence of TAC, ###(p < 0.001) indicates statistically significant difference from presence of TAC without presence of inhibitors