Skip to main content
Fig. 1 | BMC Cancer

Fig. 1

From: Up-regulation of Orai1 expression and store operated Ca2+ entry following activation of membrane androgen receptors in MCF-7 breast tumor cells

Fig. 1

Effect of mAR activation on Orai1 protein abundance at the surface of MCF-7 cells. a Original confocal microscopy of non-permeabilized MCF7 cells treated for 15–120 min with TAC-BSA (100 nM) and stained with anti-Orai1 antibody (green) and DRAQ-5 (blue) for nuclei. b Arithmetic means ± SEM (n = 6) of Orai1 protein abundance in non-permeabilized MCF-7 cells without (white bar) and with (black bars) a 15 min to 120 min treatment with testosterone-albumin-conjugates (TAC, 100 nM). ***(p < 0.001) indicates statistically significant difference from absence of TAC. c Original confocal microscopy demonstrating colocalization of Orai1 (green) and Na+/K+ ATPase (red) in MCF7 cells. DRAQ-5 (blue) indicates nuclei

Back to article page

BMC Cancer

ISSN: 1471-2407

Contact us