Fig. 2

In vitro effect on cell cycle distribution, proliferation and apoptosis. a Cell-cycle distribution of propidium stained cells after 24 (up) and 72 h (below) drug incubation. b BrdU incorporation after 24 h drug incubation. c Annexin-V detection after 72 h drug incubation. Data = means ± SEM from three independent experiments performed in triplicate. ∗Significantly different from control conditions (p < 0.05; Tukey). d Immunoblotting for (cleaved)PARP and downstream signaling molecules after 72 h drug incubation. β-actin served as loading control. Blots shown are representative for two independent experiments